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Image Search Results
Journal: Molecular Cancer Therapeutics
Article Title: Silencing of Tubulin Binding Cofactor C Modifies Microtubule Dynamics and Cell Cycle Distribution and Enhances Sensitivity to Gemcitabine in Breast Cancer Cells
doi: 10.1158/1535-7163.mct-10-0568
Figure Lengend Snippet: Figure 1. Expression levels of different proteins in MCF7 cells with low TBCC levels. A, representative blots corresponding to TBCC, a-tubulin, b-tubulin, bIII-tubulin, TBCD, Arl2, and b-actin in MC-1, MC-2, MC-3, MP6.1, MP6.2, and MP6.3 cells. B, representative blots corresponding to a-tubulin and b-tubulin in NPT heterodimers, PT heterodimers, microtubule heterodimers, and total pool of tubulins in MC-1 and MP6.1 cells.
Article Snippet:
Techniques: Expressing
Journal: Molecular Cancer Therapeutics
Article Title: Silencing of Tubulin Binding Cofactor C Modifies Microtubule Dynamics and Cell Cycle Distribution and Enhances Sensitivity to Gemcitabine in Breast Cancer Cells
doi: 10.1158/1535-7163.mct-10-0568
Figure Lengend Snippet: Figure 4. Cell cycle distribution and expression level of related proteins. A, percentages of cells in the S-phase and G2-M phase of the cell cycle. MC and MP6 represent the average value of 3 clones of MC or MP6, respectively. MP6.1 þ siRNA SCR, and TBCC correspond to MP6.1 cells transfected with siRNA scrambled and siRNA-targeting TBCC, respectively. Values differ significantly at P < 0.05 (*). Bars represent SD. B, representative blots showing TBCC content in MCF7 cells 48 hours after double-transient transfection with siRNA-targeting TBCC and scrambled siRNA. C, representative blots corresponding to the expression levels of CDK1, cyclin B, CDK2, cyclin E, p21, and p53 at basal levels and after 24 hours exposure to gemcitabine (1 nmol/L) in MC-1 and MP6.1 cells. D, quantification of the expression levels (ratio of protein versus b-actin) of CDK1, cyclin B, CDK2, cyclin E, p21, and p53 in TBCC models.
Article Snippet:
Techniques: Expressing, Clone Assay, Transfection